Plant and Cell Physiology, 1969, Vol. 10, No. 2 291-305
© 1969
Article |
Crystallization and some properties of cryptocytochrome c from aerobically-grown Pseudomonas denitrificans
Biological Institue, Faculty of Science, Nagoya University Chikusa, Nagoya, Japan
- 1. Analyses of cytochrome types in intact cells of aerobically- and anaerobically-grownPs. denitrificans indicated a higher ratio of cytochrome c to cytochrome b in the former than in the latter.
- 2. Anaerobically-grown cells contained about two times as much cryptocytochrome c as did aerobically-grown cells.
- 3. Crystalline cryptocytochrome c obtained from the soluble fraction of cell-free extracts of aerobically-grown cells manifested the same properties as cryptocytochrome c from anaerobically-grown cells, i. e., absorption maxima, autooxidizability, redox potential, molecular weight, haem content, etc.
- 4. Cryptocytochrome c was reversibly converted to a true haemochrome type spectrum by alcohols, detergents, carboxylic acid salts, guanidine salt or high pH values.
(Received December 16, 1968; )
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